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GrainGenes Sequence Report: BQ459297

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Sequence
BQ459297
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC173515
NCBI UniGene
Hv.2218
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, weakly similar to NP_001048039.1 Os02g0734400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HA
Tissue
embryosac
Developmental Stage
0-7 DAP (days after pollination)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HA01K04r HA Hordeum vulgare subsp. vulgare cDNA clone HA01K04 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HA01K04
Probe
GBM1256
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 468 Std Error: 0.00; Plate: 1 row: K column: 4; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
DNA
cggacgaggcggcctcgatctgtggaaagaagaggatagatcgggagaga
gagagagagataggaggaggaggaatcgcctgacacgatgggcgccaccg
gccaacgtcggctgtcatctctgcccgcggcggcggcggccgcgcctctc
ctgcttctgctcgccgtgtcctcctggagccaggcggcggcgtcggcggc
ggcggctccggcctggacgacggacctggagaagcacgtggcgttcttcg
acaccgacaacgacggcatcgtcaccttctccgagaccgagcaagggctt
cgtgccatcggtcttggagttctcgaggccacggccagcgccaccctcat
caacggagtcatcggacccaagaccagacccgaaaatgctacgacatcga
ggtttgacatctacatagccaacatccacaaagggatgcacggaagcgac
agtggctcgtacgacgcc

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