Query (optional)   in Class  

GrainGenes Sequence Report: BQ460950

[Submit comment/correction]

Sequence
BQ460950
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC181272
NCBI UniGene
Hv.10941
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to NP_172514.1 PP2A-2 (protein phosphatase 2a-2); protein serine/threonine phosphatase [Arabidopsis thaliana]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HE
Tissue
etiolated leaves
Developmental Stage
grown for 6 days in growth chamber (harvested at daylight conditions)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HE01K18r HE Hordeum vulgare subsp. vulgare cDNA clone HE01K18 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HE01K18
Probe
GBM1359
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 465 Std Error: 0.00; Plate: 1 row: K column: 18; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp
DNA
caccctccccgcaatagcaaacacgcggaaaaaaatggaggcatgaacgc
gccctcctcctcctcctcctcctccatccccccctcctcctccctcgatc
ccgtcgacggcccagcggccccgcccccgccccctgccccccggcgggaa
ccttgctcgtaggcggacgtgaggggagcggcgcgcggcagcgacgatgc
cgtcgcactcggatctggaccggcagatctcgcagctgcgggactgcaag
ttcctgccggacgccgaggtcaaggccctctgcgagcaggccaaggcgat
cctcatggaggagtggaacgtgcagcccgtgcgctgcccggtcaccgtct
gcggcgacatccacggccagttctacgacctcatcgagctattccgcatc
ggcggcgacacccccgacacgaactaccttttcatgggcgactacgtcga
ccgtggctactattc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.