GrainGenes Sequence Report: BQ461468

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Sequence

BQ461468

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Hordeum vulgare

Keyword

EST

Species

Hordeum vulgare

Cultivar

Golden Promise

Clone Library

Tissue

callus

Developmental Stage

callus (5-10 mm in diameter)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HD04D14r HD Hordeum vulgare cDNA clone HD04D14 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

Clone

HD04D14

Probe

GBR1681

Remark

DB_xref: taxon:4513

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 523 Std Error: 0.00; Plate: 4 row: D column: 14; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

DNA

cggcacgagggaagaatggaagagattggaagtagctattttgatgaatt
actaagcaggtccttcttccaacatcacaaaggtggatatgtgatgcatg
atgctatgcatgacctagcacagtcggtctcaattcatgaatgcctcaga
ctggatgaccttccaaacaacaacagctctgcaaaaagtgccaggcacct
ctcattctcttgtgaaaatagaagccagacttcatttgaagcctttcttg
gatttaagagagcatggacacttctactgctaagtggatataaatcaatg
acaaggtctatccccagtgatctgttcctcgaattaaggtacctccatgt
gctcgatttaaaccggcgagacattactgagttgccagattccattggaa
gcttaaaaatgcttcaatatttgaatctctcgggcactggtatagcaatg
ttgccttcatcaattggtaggctcttcagcttgcaaatacttaagttgaa
aaactgccatgaattagattatc


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