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GrainGenes Sequence Report: BQ466026

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Sequence
BQ466026
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC171703
NCBI UniGene
Hv.12675
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001051781.1 Os03g0829200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HT
Tissue
endosperm early
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HT01F21T HT Hordeum vulgare subsp. vulgare cDNA clone HT01F21 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HT01F21
Probe
GBM1164
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 645 Std Error: 0.00; Plate: 1 row: F column: 21; Seq primer: T3.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
aatgtgtagacaactcgggcggctgtcagaggcatcttccacagtgacgc
agtggcatgtgctgtgtgcattgtatagtacgtactattagtatagttga
acagtgcagtggtatttttactgtttgcgcccggccatgcacgtgagttt
attcggtctcgccattattaattccggccggcaaaaaggagagaacaaaa
ctgcactgttccgatcgtcagatcatcatcatcatcatggttgattcagt
ggattaagagtgaataatccattttctaagagtaattttgatatgatgga
tggatgaaggtgttcgtggtgggtcacgactggggcgccatcatcgcgtg
gtacctgtgcctcttccggccggaccgggtgacggcgctcgtcaacacca
gcgtcgccttcatgcgccacgtcttcatccgcgccggcgccggcgccgtc
aagaccaccgactacttcaaccaagcctacggcccgacctactacatctg
ccgcttccaggtctcctcctccgctcctctgtctatactaccgactccga
cgacgtctggactaaccatcgacgacggcgacgacgacgtggtgcatgag
cccggggtggcggagaaggagttcgcgccggcgcacgcgaggcac

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