Query (optional)   in Class  

GrainGenes Sequence Report: BQ466375

[Submit comment/correction]

Sequence
BQ466375
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC162610
NCBI UniGene
Hv.7214
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf35i20, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HT
Tissue
endosperm early
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HT02F17r HT Hordeum vulgare subsp. vulgare cDNA clone HT02F17 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HT02F17
Probe
GBM1365
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 560 Std Error: 0.00; Plate: 2 row: F column: 17; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgaggcttcccacggcatacacgaggcggcggcggcggcggcggc
ggtgatgggaggcgagggcgccagttcaagcggcggggggttccgcgcca
ggatagatcacgtcctgtacagcggagagaagaagcacgtcgtcgcaggc
atcgccatcttcgccgccattttcggggttccctggtacctcatgacccg
aggggcaaagcatgactctcatcaagattatgtggagcgagctaacaagg
cgaggtcggataggctttcttctggacagccatcactgaaagaatgaagg
agctgtctcagccggccccagattacccaacctctgcatatgcagtttgc
attatgaatctgaaggcttaatcttgtgacaaataaacagaccttgtatg
ttcctcgtgtcggcagccatgggagaaacgtagtgttattgtaatacagt
aacagtagaaattttgttcgcggaaatctcatagtagtcttttttgctat
tctaggaggagacgatggacaccaaatcatgatatttttgttgcacgaat
tgcagtccgg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.