Query (optional)   in Class  

GrainGenes Sequence Report: BQ468279

[Submit comment/correction]

Sequence
BQ468279
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC169006
NCBI UniGene
Hv.22899
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Carbonic anhydrase'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HP
Tissue
epidermis
Developmental Stage
seedlings grown in greenhouse for 6 days
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HP01L24T HP Hordeum vulgare subsp. vulgare cDNA clone HP01L24 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HP01L24
Probe
GBM1425
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 554 Std Error: 0.00; Plate: 1 row: L column: 24; Seq primer: T3.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp
DNA
cggcacgagggctaactggtgctacgcaaccgtcgcgccccgtgcccgct
cctccaccatcgccgccagcctcggcacccccgcgccctcctcctccgcc
tccttccgccccaagctcatcaggaccacccccgtccaggccgcgcccgt
cgcacctgcattgtaatccgtccgtccatccatccggggagagaagagag
aagagagaagagaggagagaaaacaaaagagtagagtcgcgatagttgaa
gatgagcggctgcctgtgcctcccctgctgctataagaagtcccccgccc
ccgcccccgctggggagaacaacaaaccagcagcggcagcggcagcggca
gcggcagcgatagattcttcctcctcgtcactcatacagaagcctaccta
ccatccaccacctcctccaccctccaacaagatggacgccgccgtggagc
gcctcaagaccgggttcgagaagttcaagaccgaggtctacgacaagaag
cccgatttcttcgagccgctcaaggccggccaggcgcccaagtacatggt
gttc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.