GrainGenes Sequence Report: BQ483003
Sequence
BQ483003
Contig
NSFT03P2_Contig7357
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Blanco
Clone Library
Secale cereale anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
WHE1287-1290_N06_N06ZY Secale cereale anther cDNA library Secale cereale cDNA clone WHE1287-1290_N06_N06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1287-1290_N06_N06
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
cagctgtaaaagcaatatccccttgagtactcaaagatacaatgaactga
gcgatacacttccaaatggaaatgaactgacgattgaacaactcatagac
cacttccaagagaatcctacaaagttaggctctcaggatcctcaacaatt
ttcgcaaaggtttgcaggaatgctgccaaatcagcaccataaacaatcct
gtgatcagctgtgacattgaccatcattttgctcttaacactaaagtaac
catctttatcagccaccactgtagattttgaggctccaacagccattata
gctccctggccaggtggaagaattgcatcaaatctatctacaccaaacat
gcccaagttggacagtgtaaatgtcccagagttgtactcattcggttgaa
gctgttttgcgcgtgccttcttgactagttccttccacttttgtgagagt
aaatatatatccagcttatcagcttgctccaggacgggtgtgatgagtcc
ccatcaatagatacagcgacagcaatattgatagaactgttgtaagtgaa
actcgtcccatccctgcagctagcgttcaccacag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ483003
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||