GrainGenes Sequence Report: TaAffx.83813.1.S1_at
Sequence
BQ484063
Contig
TaAffx.83813.1.S1_at NSFT03P2_Contig11964
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC297019
NCBI UniGene
Ta.1503
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001060120.1 Os07g0584500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed root cDNA library
Tissue
Roots
Developmental Stage
Full tillering
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3516_A04_B08ZS Wheat unstressed root cDNA library Triticum aestivum cDNA clone WHE3516_A04_B08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3516_A04_B08
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab ). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California , Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California, Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tcggcacgaggtatcagcaggtttccaacttcttgaatggcttcaaccag
ggatatagcccaaacccgataggaggttacggcatgagagtggatggaag
gtttgggctgctttcaggtgcacgaaatgggttttcttcatttggcccca
gttatggaatgggcatgaatgttgaaactgggatgaatgcgaattttggt
gcaaactctagtttcctcaataactcaaatgggcggcaaatgggttcata
ctacaatggtggttcaaacagactaggcagccctattgggtatgttggtc
tgaatgacgattcaggatcaatattgagttcaatgggaaggaatgtttgg
ggtaatggaaatgtcaactaccagaacagccctacaaacatgagttcttt
tgtaccatctggaagtgggagtcaagttggtattactggcgacggtataa
attggggaggtcctacttctgcccacgggatgggaagcatttcaagcctt
gggtctaacattggccgtggggctggagataactttggcttgccgtctgg
tggctatggaaggagcaacccaactggcaccattggtgaacctttttctg
cgtcagccaatgcatat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: TaAffx.83813.1.S1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||