GrainGenes Sequence Report: BQ487466
Sequence
BQ487466
Contig
NSFT03P2_Contig7561
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC362978
wEST map position
BQ487466
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6AL 6DL
Clone Library
Wheat salt-stressed crown cDNA library
Tissue
Crown
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2112_H08_P16ZY Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2112_H08_P16, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2112_H08_P16
Probe
WHE2112_H08_P16
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
aaaaaaactttgtacactgtagctcaactagagtctacagctaacatcca
tccatatatcatcaactccgcagcaaggccttacgcgtccggccgcagga
tatgtaaatacaagtttcaaaattcaaaattttagtttgcccagttcaaa
gttcatccaactgtcgcctgggatgcgcctcatcaagccctctctcaggt
gaaccaaccatctttagctcctatagatagatgtacaagccacattgagt
tgacaaacaacaacagcatgatcctgcggtgcaatgcaggtgcgccggcc
agcgcgcctgctacccgatgttggccacaaacggcaccaggcgcttgtgg
tcccgcttgcggccgagggcacaggtat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ487466
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||