GrainGenes Sequence Report: BQ487515
Sequence
BQ487515
Contig
Ta.7738.1.S1_at NSFT03P2_Contig14987
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ487515
DB Remark
Locus Source: Triticum turgidum
Keyword
EST
Species
Triticum turgidum
Cultivar
Langdon-16
Chromosome
1AL 1BL 1DL
Clone Library
Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library
Tissue
All tissues
Developmental Stage
Different growth stages
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2160_D08_G16ZY Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library Triticum turgidum cDNA clone WHE2160_D08_G16, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2160_D08_G16
Probe
WHE2160_D08_G16
Remark
DB_xref: taxon:4571 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttatttattgttcaaagagataccctagacaaagaaacagaaaagaacg
aggaaaaatctgtacagggtgccgaaaggaagctagaacaaaggtctcag
tctcaggggatgatgatcacgcatgatcaatcagcactgctctttctcta
ccgcctacaatgcaaaatgaagcgcaatgcaatgtcctacaggaaatgct
catgctatctctactaccctcggccagagccgagttctcggggacgccct
cgttcgtcgacaccgatcttcatggctggaacctcgccgagggccccgag
tcgccgctcacctcgaactcgctcgagctcgccttggactcggcgtccgc
gttgccaccggcatggccgcccctccggctcctccggtcctcccgtgatg
ggacatcctctgcctcaagcatccggacaacatggcccatagtgggtctc
ttctcggagtccgggtcgacgcacctc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ487515
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||