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GrainGenes Sequence Report: BQ659219

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Sequence
BQ659219
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC193233
DB Remark
Locus Source: Hordeum vulgare
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HD01H16w HD Hordeum vulgare cDNA clone HD01H16 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD01H16
Probe
GBM1087
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 681 Std Error: 0.00; Plate: 1 row: H column: 16; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
gaagagcaataatactttactttcgacaaagggtgaattttattggttta
aaatggagcatcaagacgatacaacacaatgaatatacacacggtctctg
cataactaagatgcacacgtccaaccaaacatacacacacaaaaacgaaa
gaagaagaacttgagtgagtaaccaggaggaggaggaggcacctttgccc
aagagagagtagctagggcacctttgctcggccggatcacacggaacggc
agcctctcttgcatggttttgccgccggccgaccgagctgtgaggtgtat
cagccgtgaagcctgtgagttgagttggaagtttgaaagctggaacggat
gggtttcttttaaacctgtgcaagcaaagcaagctgagcgtccagcatgc
tcacacctgcaaaaatattctcacgcacggcattttattaaaagtctaaa
gcagacaagaatagacctcgccacttgggcttagtgctttcttttacacg
cacatacctcactttatcacgcgcggcagcatatagaaaaagaatttatt
tatttattgattttacgatcaacaaacttgacgaatggtgagttggtaaa
gcgagcatgacttatcaccagacaagacttttaagaagagcattagcaac
ttgttgtgcatggtgcgctgtctgaatcatg

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