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GrainGenes Sequence Report: BQ659365

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Sequence
BQ659365
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC181925
NCBI UniGene
Hv.21127
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001060737.1 Os07g0694300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HE
Tissue
etiolated leaves
Developmental Stage
grown for 6 days in growth chamber (harvested at daylight conditions)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HE01A24u HE Hordeum vulgare subsp. vulgare cDNA clone HE01A24 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HE01A24
Probe
GBM1214
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 353 Std Error: 0.00; Plate: 1 row: A column: 24; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp
DNA
ctctaaaaatggcacgttagcgcttggggcatacgttgggaactacagcg
aattcggataacgcatctccatcaggcatgcatgctctgctcatgctaca
gcaagcatgcacaaacacatacacacccatgttcccttcatcacttccga
atcattagcgcccctttccacacacacacacacacacacacacacacacg
atcaggaagcatgtacgtcttgtcactgagacagtgatcaccagtgatca
cttggcgttgacgaacctgcagtccgtcctgacctccccctgcccgccgg
tgagcggcccgttctccgacatcctcaccagcgccgccgagaacgcctcg
cgg

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