GrainGenes Sequence Report: BQ659846
Sequence
BQ659846
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC157774
NCBI UniGene
Hv.8616
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Peptidase of D1 protein'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HF
Tissue
caryopsis
Developmental Stage
developing caryopsis, 16-25 DAF (days after flowering)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HF01O14w HF Hordeum vulgare subsp. vulgare cDNA clone HF01O14 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HF01O14
Probe
GBM1094
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 668 Std Error: 0.00; Plate: 1 row: O column: 14; Seq primer: T7. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp
DNA
gaataaatacatgtataattccataataaggtattccaaatattagcaat
tcttagtaatttgaatatagtggcaggacacataatttgaacaatcacaa
gctatttacatattggtgctgggtataatcggcagaagccaatgcaatca
agggctctctctctctctgtaccgagctgcagcacctgtgaatgcaatcc
aagggtatgcacactctgatctctgcagaagcctatattgatgcttcggc
gtagagcttgtgacctcacctcaccagggagagttcattcgtcactgtac
aattctcccaagcataaccctccgttccatacttctaatgtgttgcctca
acttaatttcttgctttggcaacgcaaaaggtaatctctggcttcattga
gatcaggaagacggcggtaatctggttttattccatctccattgatgtct
ttatgatttggtgtgacatacttgccaactgtgacaacaataccagaacc
atcatgaagttcaaatacagattggattaagcccttgccaaaagtccttt
cgccgacgagaacagctttgcaattatcatgaagtgctgaagcaactatt
tcgctggcacttgctgtcctattattcactaataccattacaggagttgt
aaccaaaggtccactgtc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ659846
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||