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GrainGenes Sequence Report: BQ659999

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Sequence
BQ659999
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC157063
NCBI UniGene
Hv.6637
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to NP_565786.1 LHB1B2 (Photosystem II light harvesting complex gene 1.5); chlorophyll binding [Arabidopsis thaliana]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HG
Tissue
green leaves
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HG01I24u HG Hordeum vulgare subsp. vulgare cDNA clone HG01I24 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HG01I24
Probe
baak28n19
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 526 Std Error: 0.00; Plate: 1 row: I column: 24; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 800 bp
DNA
aagcaatttcccaaaagatccttgtaatttacatcatgatgttacaaaac
cacacaatacgtgtgcagttcactgtagtcgccttaatggctacaactcg
cagctaactagcgccaacgcctcacttgccgggaacaaagttagtggcaa
acgcccatgcgttgttgttgacaggatcggcgagatggtcagcaaggttc
tcgagggggcccttgccggtgacaatagcctgcacgaagaagccaaacat
ggagaacatggcaaggcgcccattcttgacctcttttaccttgagctccg
cgaatgcctctgggtcatcggctaggccgagcgggtcgaagctgccacca
gggtagagtggatcaaccacctcaccgagtgggccaccagcaatgcggta
cccttcaacggcgcccatgagcacgacttgacatgcccagatagcaagga
tgctttgtgcatgcacgaggctagggttgccgaggtagtcgaggccaccg
tcgctgaagatttgggagcctgcctt

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