Query (optional)   in Class  

GrainGenes Sequence Report: BQ661852

[Submit comment/correction]

Sequence
BQ661852
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC154294
NCBI UniGene
Hv.8948
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001068430.1 Os11g0671000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HP
Tissue
epidermis
Developmental Stage
seedlings grown in greenhouse for 6 days
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HP01H24w HP Hordeum vulgare subsp. vulgare cDNA clone HP01H24 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HP01H24
Probe
GBM1142
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 566 Std Error: 0.00; Plate: 1 row: H column: 24; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp
DNA
agaaagaacttgctctgaggctgttattatcttaccatagtcggttaaac
attgagaagaccacaccacccacacgagagaatttacacatggagcgcac
acaaagtacacacatacacccatcactcgctagcatgcggcgagcaaaga
gcaaactgaggtaacaaaagaaagttacaagcactagtatagtagtacta
ctaagcttaggagaaactacatgcagcctcggacagcaacgtacgtacgc
aggatttagataaccaaaagagcagcaaacatggcgtcgtcccagatcga
agttgtcgatcgagcacccccgtcttcctcttctttagcggtggttgctc
ctggtcctggtctcgtcgctgtaaagccagtcgtagacggtgggggagtt
gggctgggggcggtcgaagaggttggcgccgaggcccttggtggcgaggt
tgctcccggggtggaagacgctccgccacacgctgccgccgcgcggcggc
gtcgacgacgacgacgacggcgtcaccggcgtggtcggggtcgtcggcat
cgactgcgacggcttc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.