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GrainGenes Sequence Report: BQ663001

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Sequence
BQ663001
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC189486
NCBI UniGene
Hv.9925
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001044324.1 Os01g0761500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HT
Tissue
endosperm early
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HT01D09w HT Hordeum vulgare subsp. vulgare cDNA clone HT01D09 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HT01D09
Probe
GBM1162
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 526 Std Error: 0.00; Plate: 1 row: D column: 9; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
aactagaaacatgtgacatttttttaatgatgatacagaccgggtgcatc
cgtcgtttctacactagcgtagcacatccctatcacaattcagcaattcg
caagagagaagaaaggcgcgcgccgaggtcaaacgggcgaacgccttgcc
tatcatttgtgttgttcttgcttcttctgttcctcctcctcctcctcctc
agccgccaatgcaatcactctgctctgcccttcctcgccgtacgcccagc
cgcaccagacgaagtacaccaagttggccacggacagcgccgcgagaacc
aggtagtagtagtcgtagcgacccctgttgaggttgctcgacaaccagct
gttgcgcccgtccctcccggtggtgctgttcatgatccccacgatggcgc
tccccagcacggcgccgaagcccaagccgagggccagcagcgacaccccg
atgctggacatggtctttgggaactcggagtagtagaactcgatctgccc
tatcaggttcaacgcgttggcgagcc

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