GrainGenes Sequence Report: BQ665018

[Submit comment/correction]

Sequence

BQ665018

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus, moderately similar to NP_001056347.1 Os05g0566900 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

apex

Developmental Stage

apex (3-5 mm in size)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HX01N01w HX Hordeum vulgare subsp. vulgare cDNA clone HX01N01 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HX01N01

Probe

GBM1278

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 380 Std Error: 0.00; Plate: 1 row: N column: 1; Seq primer: T7.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

gaaaaaagcacctgttaggtaaactatgtctccaaggagttacaagactg
ttctgctccgcgcatataacgaccgaaaaacaaacacatcagtccgacaa
caccgacatgtgacacgcaacacgttaaaacgctctgccaagacgacgcc
gaccctctcctcagcgagccacaagctcagctactctgagctgctttact
ccgcagccacagtgagccagcaccgcaacaagagtgcgggcggctcagta
gctggtgtgctgctgccagctctggtgagatggctgctgctgctgctgct
gctgggctgccgcctggtagccgttcagggttccgtccgccgggttctga
gggtggtgctcctgcgccaagcctccctgg


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.