GrainGenes Sequence Report: BQ666000

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Sequence

BQ666000

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf62c24, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

pericarp

Developmental Stage

0-7 DAP (days after pollination)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HZ01N06u HZ Hordeum vulgare subsp. vulgare cDNA clone HZ01N06 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HZ01N06

Probe

bags1a18

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 603 Std Error: 0.00; Plate: 1 row: N column: 6; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp

DNA

aggaggctaaggcatagataaccagatagtaacacatattgaaacaggaa
aatggagacagaggccaaggcatagataaccgcatgcacataacattagg
ttcagacagaccaatatgttcttacaacacaacaggttgtaaaacaaaca
ccacaaagcaaacattcaaacagacagataacagataaagccgcttgggc
agatcataggatagcagtagtacgccttgggaaagcaccaggaggcaggc
tctagtactcatcaccctcgtcaccatcctcaccatcgtcgaactcagca
ccaacttcttcatagtccttctccagggcagcaaggtcctcacgggcctc
agagaactctccctcctccataccctcacccacataccagtggacaaagg
cacgcttggcgtacatcaggtcaaacttgtggtcgatgcgggagaagacc
tcaacaacactggtggagttggagatcatgcacacagccctctggacctt
ggcaaggtcgccccctgggacgacaccaggtggctggtagttgataccgc
acttgaagccagtggggcaccagtcaacaaactggatagtgcgcttggtc
ttg


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