GrainGenes Sequence Report: NSFT03P2_Contig10746
Sequence
BQ805568
Contig
NSFT03P2_Contig10746
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC340911
NCBI UniGene
Ta.10544
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001068204.1 Os11g0594200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Butte 86
Clone Library
Wheat developing grains cDNA library
Tissue
whole grains
Developmental Stage
3-44 days post anthesis seed
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3568_E07_I14ZS Wheat developing grains cDNA library Triticum aestivum cDNA clone WHE3568_E07_I14, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3568_E07_I14
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Plants were grown under six following different environmental regimes in greenhouse, Environment 1) 24oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 2) 24oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 3) 37oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 4) 37oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 5) 37oC/17oC day/night plus drought, with post-anthesis fertilizer, Environment 6) 37oC/17oC day/night plus drought, without post-anthesis fertilizer, developing wheat grains from the following were excised and frozen in liquid nitrogen, Environment 1 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 2 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 3 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 4 at 3, 5, 7, 8, 10, 12, 16, 20, 24 , 28, 32, 34 DPA Environment 5 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA Environment 6 at 3, 5, 7, 8, 10, 12, 16 , 20, 24, 28, 30 DPA and total RNA was prepared by S. Altenbach and K. Cronin at USDA-ARS, Albany, CA. A cDNA library was made using poly (A) RNA, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (others). Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Plants were grown under six following different environmental regimes in greenhouse, Environment 1) 24oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 2) 24oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 3) 37oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 4) 37oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 5) 37oC/17oC day/night plus drought, with post-anthesis fertilizer, Environment 6) 37oC/17oC day/night plus drought, without post-anthesis fertilizer, developing wheat grains from the following were excised and frozen in liquid nitrogen, Environment 1 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 2 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 3 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 4 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 5 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA Environment 6 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA and total RNA was prepared by S. Altenbach and K. Cronin at USDA-ARS, Albany, CA. A cDNA library was made using poly (A) RNA, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (others).
DNA
tggctagtggttcggcgacatcactgttcgcttctgggacctcagcacac
agaccccattgtacacatgcaaaggccacaagaattgggttctctgcatt
gcatggtcacctgatgggaaacatcttgttagtggaagcaagtcaggaga
gcttatattatgggaccccaaaacaggcaatcaattgggcactcctctta
cgggacatagaaagtggattactgctgtatcttgggagccagttcatttg
caatctccttcccgtcgttttgtaagtgcaagtaaagatggcgatgcacg
aatttgggacattaccacaaagaagtgtgttatttccctttcaggtcaca
ccaatgctgtgacctgtgtcaagtggggtggagacggtttgatatataca
ggttctgaagattgtttaatcaaagtatgggaaactactcagggcaagtt
ggtcaaaacactgcagggtcatgggcactgggtaaattcgcttgccttga
gcacagaatatgttctccgcactggggcatatgaccataccggcaagaca
ttttcaagtccagaggaaatgaaagaggcagctcttgcaagatatgagaa
gatgaggggtaatgctcctgagaggctggtctctggttcagatgatttta
ccatgtttctttgggaaccaacgattagcaaacagccgaaagctcgcatg
actggtcatcagaaggtggtgaaccatgtctacttctcccctgacgggca
gtggctggcgagtgcctcctttgacaatcggtcaagctgtggaatggcat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig10746
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||