GrainGenes Sequence Report: Ta.23795.1.S1_at
Sequence
BQ838094
Contig
Ta.23795.1.S1_at NSFT03P2_Contig16172
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC361930
NCBI UniGene
Ta.54637
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001058666.1 Os06g0731800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
BH1146
Clone Library
Wheat aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2906_E02_I04ZS Wheat aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE2906_E02_I04, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2906_E02_I04
Probe
MAG1933
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
attcggcacgaggagaaaggacaggcaagaaggacaaggacaagaagcct
gggatcatcgtggtccagggccccaagcccgggaaggccaccgacatggc
acggatgcgccagatcctgtcgaagctgaagcacacaccgccgccacaca
tgaagccacctccaccacccgctgttgcacctgccgggaaggacggagct
ccggccgccgctggaaaggacggagctaaggcggcagcgacacctgctgc
caaggatgcccctgccaacggcactgtccctgagaaggagaacgcagcac
ccgccgtcgctgccgccccagcagcagcagctccggcgccaccagctgag
cccatcgccgctgcctgaacgaggatggcttcggtcagccgctaatcctt
cgcagtagggttatatatttgggtgtcagtagagttgttccaggagagac
ggggaaggagcgtgaaggccatatacgatgcgaggtggtgaaatcgtctg
agttcaccacggggaatcatggcggagacacacgcagtgtactattatta
atttgagtttgttattatgagcaggagggttgttacttatgggaccagca
aactttattcgttaatattgtcattgcattttgtagagagaaagagggga
ctggtaagaataaggtaaggctgtgctgtatgtgtttgtatatttatcat
caattgttggatttttggtaa

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.23795.1.S1_at
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