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GrainGenes Sequence Report: Ta.20550.1.A1_at

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Sequence
BQ903721
Contig
Ta.20550.1.A1_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC340041
NCBI UniGene
Ta.18397
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Flavonoid 3'-hydroxylase'
Keyword
EST
Species
Triticum aestivum
Cultivar
FHB148
Clone Library
Ta03_AAFC_ECORC_Fusarium_graminearum_inoculate d_wheat_heads
Tissue
heads
Developmental Stage
anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
Ta03_18a03_R Ta03_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_heads Triticum aestivum cDNA clone Ta03_18a03, mRNA sequence.
Clone
Ta03_18a03
Probe
MAG3601
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Ouellet, Therese; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A 0C6,; CANADA; Tel: (613) 759-1658; Fax: (613) 759-1701; Email: ouellettr@em.agr.ca.
Locus Comment: Contact: Ouellet, Therese; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A 0C6,; CANADA; Tel: (613) 759-1658; Fax: (613) 759-1701; Email: ouellettr@agr.gc.ca.
Note: Vector: pGEM-T easy; Site_1: EcoRI; Site_2: EcoRI; Controled chamber-grown wheat heads were spray inoculated at mid-anthesis with a Fusarium graminearum macroconidial suspension (50,000 spores/ml) and kept under intermitent misting for 24 hours, then collected and immediately frozen in liquid nitrogen.
DNA
rrtacaccratrrcartaacctcggcacgggctgaaccattarcggcacg
gcccgctgcaaagtgaaaccatargcctcctccatgtcgagcttttcagg
tgtcatgccatcaaccaaggtccagtcgaacgcgtgcacgagcgtggcgg
tcatgagtgtgaccatccggaggccccagctgaggcccgcgcatatcctt
cgccccgccccgaatggaatgagctcatagtcgccccccttgacatccac
gg

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