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GrainGenes Sequence Report: NSFT03P2_Contig9440

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Sequence
BU100414
Contig
Ta.7657.1.S1_s_at
Ta.7657.2.S1_a_at
Ta.7657.3.S1_a_at
NSFT03P2_Contig9440
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC278494
NCBI UniGene
Ta.7657
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001060917.1 Os08g0129700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Chinese Spring aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE3353_A10_B19ZS Chinese Spring aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE3353_A10_B19, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3353_A10_B19
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown for four days under hydroponic conditions with 1ppm aluminum, root tips were excised and snap frozen, and total RNA was prepared at University of Missouri in the JP Gustafson lab (Ross). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ggcacgaggattttggaggctatggcttctcatggagtcaagacacttat
ttactctagtacctgtgcaacttatggagaaccagagaagatgcctataa
tagaaaccacacctcagttgccaattaacccctatggaaaagccaagaaa
atggcagaggacatcatactagatttctcaaagagaacagatatggctgt
catgattttaagatatttcaatgtcattggatcagacccggagggaagat
taggtgaggctcctaggcctgaactacgagagcatggaagaatatctgga
gcatgctttgatgcagcattaggagtcatttcaggattgaaggttaaagg
gatagattatcctacagctgatggaacctgcatacgagactacattgatg
tcacagatcttgtagatgctcatgtgaaagcactcaacaaggcagagcct
agtaaagttggcatttataatgttggcactggaagaggccgttccgttaa
ggagtttgtcgatgcctgcaagcaggcaactagggttgacatcaaaattg
agtacctcagcaggaggccaggagactatgctgaagtattcagtgacccc
accaagatcaacagtgagctcaactggactgctcaacataccgatctgaa
ggaaagcctgtctgtcgcgtggaagtggcaaaagtcgcatccgcggggct
atggggcaaactagatatagctcgcaatcaataactccgctgaaatcgtt
aatacatgttgattctgtagtta

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