GrainGenes Sequence Report: BU101049
Sequence
BU101049
Contig
NSFT03P2_Contig11296
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC301024
NCBI UniGene
Ta.22911
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001064245.1 Os10g0177400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Chinese Spring aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3363_D12_G23ZS Chinese Spring aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE3363_D12_G23, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3363_D12_G23
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown for four days under hydroponic conditions with 1ppm aluminum, root tips were excised and snap frozen, and total RNA was prepared at University of Missouri in the JP Gustafson lab (Ross). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tcggcaccaggtgctacaagactgttgaactgaacaacctgagatcgagc
aactcatattctggtgtcagtactggattttagttgcccttatgacggtt
atggagaggtttggagatttaacaatatcatggctaccattttactcaga
ggcaaagctgctgttctttatatacttgtggtaccccaggacaaagggga
ctacctacatttatggaactttctttaaaccatacatttcacagcatgag
aatgaaattgaccgtaatttgcttgagttgagagctcgggccagcgatgt
tgttgtcgtttatgtccagaaggttgctgctgttggacagaataccttct
ttgatgttttgaagtatgttgcgtcacagtcaccatcccagaaatcaaag
caacaacgtcctcaggaaccacagcagtcacaaccacaaccacaggtaca
gcagcagcaaccacaaaggcaagcgcagcctgttatgcacagagctgcat
ctattgctgcacggcaagcagcaatggcacagcaagctcaagagaccaag
cccatttcccccaagttcaagcgtcaagcatcagcaaaaggtggtccagc
ggcatccacgaaacccgccgcggctgctgcgcccacaacaaaacccgatg
ag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BU101049
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||