GrainGenes Sequence Report: NSFT03P2_Contig12277
Sequence
BU101138
Contig
Ta.15800.1.S1_at NSFT03P2_Contig12277
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC337338
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Chinese Spring aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3363_A05_A09ZS Chinese Spring aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE3363_A05_A09, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3363_A05_A09
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown for four days under hydroponic conditions with 1ppm aluminum, root tips were excised and snap frozen, and total RNA was prepared at University of Missouri in the JP Gustafson lab (Ross). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
attcggcacgaggaaacatgcacgatttgtcatacctgtcttggctatgt
atgctgtttattgtgtggctgttcgtgtcgggtggcttggagttttcttc
acgctgaatctctcttttctgacaaatgatcttctcaacaagttgctgca
aggatatgagggaagcacggaagagagaccatttgaagaaatgaaggagt
ctgatccagccacggatgccttctttcgtggctgtgaatatcctcctgct
cctgaaagtgaaccagagactgtgtcgtctgcaaagcccttttgcgcagc
acctacccaagatgtgttacatgtacagaaggagccatctcctagtaaaa
tagtgaaatcaaattctacttcgttggacgagatgaagaggataatggat
ggttcgacctattatgatgttttgggcatccctcggagcaaaagcatcaa
tcagatcgaattgaaaaaggaatacagaaaactggtaatagacaacaaca
ctttaagctgaacgatcatccatatttctgttccttattgtcctggtgca
ttttgtctgtctaggcagttcttgtccatcctgataaaaacatggggaat
ccattggcgtgcgagtcattcaagaagcttcagtcagcttttgaggtact
ctcagatttaacgaagagaaatggctatgatgaacagctgaggaaagaag
aatcgcggcaaatgactcagagatcacgtgttgtctctc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig12277
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||