GrainGenes Sequence Report: BU973156

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Sequence

BU973156

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus, strongly similar to NP_001062103.1 Os08g0487800 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

developing caryopsis

Developmental Stage

8-15 DAP (days after pollination)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HB23O24r BC Hordeum vulgare subsp. vulgare cDNA clone HB23O24 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HB23O24

Probe

GBR1543

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 619 Std Error: 0.00; Plate: 23 row: O column: 24; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

cggcacgaggagaaactgaagagacagcagagggcgagaaggaaaagaag
aagaaaactatcactgagaagtactgggattgggaattggcaaatgaaac
aaagccaatatggatgagaaatccaaaggaagttgaggaaactgagtaca
atgaattttacaagaaggcattcaatgagtttttggatcctctagctcat
gcacacttcacaacagagggtgaggtggaatttaggagtgttctctacat
cccaggaatggcaccgcttagcaatgaggagataatgaaccctaagacca
aaaatatccggttgtatgtcaagagggttttcatttctgatgactttgat
ggcgagctgttccccaggtacttgagctttgtcaaaggtgttgttgactc
gaatgatctccctctgaatgtttctcgtgagatccttcaagaaagtcgta
ttgtcaggatcatgcgcaagagacttgttaggaagacttttgatatgatt
caggatattgctgacaaggatgagaaggaggactacaagaaattttggga
gagctttggcaaatttatgaaacttggttgcattgaggactcaggaaatc
agaagcgccttgctccttt


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