GrainGenes Sequence Report: BU973302
Sequence
BU973302
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC168880
NCBI UniGene
Hv.6156
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf107e14, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
BC
Tissue
developing caryopsis
Developmental Stage
8-15 DAP (days after pollination)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HB24H09r BC Hordeum vulgare subsp. vulgare cDNA clone HB24H09 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HB24H09
Probe
GBR1474
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 426 Std Error: 0.00; Plate: 24 row: H column: 9; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgagggccaagttcctaaggaagctgacagaggaggagatcacat
tcatcgcccaatcggcagccaactacatcaacttagctcaagttcatgcc
actgagaattccaagagcttcgacgagattgagctcgagaagaagttgag
gaagaagtttgctcacaaagacgaggagtacgactctatgctcggaatag
tccgtgagatcccgccgcagctcatcctccccgacaacatcctcccagac
aaggcaccgaaaacagctgccgtcgctcgctgagatcaaacccctcgtag
ggatgctttggtttttgttacatggcgacttggaacatttgacgtatgtt
ttggtcttgttctctgttttttcatgcccaataagattttgggtcgagcg
ctgttgtaggatcgaccgtatacagt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BU973302
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||