GrainGenes Sequence Report: BU978857

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Sequence

BU978857

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf123j21, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

embryosac

Developmental Stage

0-7 DAP (days after pollination)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HA14H14r HA Hordeum vulgare subsp. vulgare cDNA clone HA14H14 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HA14H14

Probe

BaGS20G24

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 558 Std Error: 0.00; Plate: 14 row: H column: 14; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

DNA

cggcacgagggagagatcgcggaggggaggaggatgacgggagggcgcgc
ggtgctcctgctgccgctgctggtgatctcggcgctgttcgcgcagatca
gcgcgtcggatccgctgttccacgagtccttcgacgagagcttcgacggg
agctggatcgtgtccggcaaggatgagtacaaaggtgcatggaagcactc
gaagagtgatggccatgaagactatggcctccttgttagtgagccagcca
ggaaatatgccataatcaaagagcttgattaccctgttactttgaaggac
aagacagttgtcctgcagtttgaagtgaggcttcagaatggccttgagtg
tggaggtgcttatatcaagtacatccgtcctcaggctgtgggatggaacg
ccaaggactttgacaatgatactccatacacaattatgtttggtcctgac
aagtgcggttcaacaaacaaggttcacttcatcctgaagcacaagaaccc
taagactggcaaatatgttgaacatcacctcaagtccccgccctctgtcc
catatgac


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