GrainGenes Sequence Report: BU983281
Sequence
BU983281
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC154075
NCBI UniGene
Hv.87
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf126g05, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HA
Tissue
embryosac
Developmental Stage
0-7 DAP (days after pollination)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HA29C20r HA Hordeum vulgare subsp. vulgare cDNA clone HA29C20 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HA29C20
Probe
bast21B1204
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 434 Std Error: 0.00; Plate: 29 row: C column: 20; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
DNA
cggcacgagggagagaactagcagtgcccaggcaagaacctggtcgtgct
cgtcggccggctcctggtggtggagctgttcctccggtacgacaccttca
cggccaaagtcggcctcgaccttctcggcaccaaggttgagttcaccggc
gtcaccaaggcaacgtccggtgtggcagacgctgtttaaatccttcaccg
gacggccatcggtgacaaagggatcaacgcagggcagcaacgatccttac
accgtcatcacgtacgtacgtacgtgcctcgctatatctaccttagtagt
gtacgtacgagtcagtcatgcatgtgcatgcatcttgttcggttgcttcg
cagcattgtgccctcgtgtatgtgtgcgtgtgttgtgtttacttttggca
atcttgcccagtgtaataagccggacttttctac
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BU983281
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||