GrainGenes Sequence Report: BU987774
Sequence
BU987774
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC165884
NCBI UniGene
Hv.22843
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, moderately similar to NP_001045270.1 Os01g0927600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HF
Tissue
caryopsis
Developmental Stage
developing caryopsis, 16-25 DAF (days after flowering)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HF15L16r HF Hordeum vulgare subsp. vulgare cDNA clone HF15L16 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HF15L16
Probe
GBR1486
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 591 Std Error: 0.00; Plate: 15 row: L column: 16; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp
DNA
cggcacgaggggcgagcttcgtgttggtgttaggcgggctatgagacagc
tgtccacgtgccttcttcagtcatttctagtcatagcatgcatcttgggg
tccttgcaactgcatggcacgctatcaacacgaaaagcatgttcacggtc
tactacaaacctagaacgagcccttcagagttcattataccatatgatca
atatatggagtctgtgaagaacaactattcaattgggatgagattcagga
tgaggtttgaaggcgaagaggcaccagagcaaaggtttactggtactata
gttggcagtgaaaatcttgaccaattgtggcctgaatcaaactggagatc
tcttaaggtgcgttgggacgagccgtcaactattccacgtccggatagag
tctctccttggaaaatagagccagcttcatcgcctcctgttaacccgctt
cctctttctcgggtcaaaagacctagaccgaatgttcctccagtttctcc
tgaatcgtctgttcttacaaaagaaggtgcaactaagattgatatggatt
ctgcccaagcacaacaacgaaatcaaaacagcatggtcttg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BU987774
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||