GrainGenes Sequence Report: BU990074
Sequence
BU990074
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC158855
NCBI UniGene
Hv.511
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf66n14, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HF
Tissue
caryopsis
Developmental Stage
developing caryopsis, 16-25 DAF (days after flowering)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HF23P23r HF Hordeum vulgare subsp. vulgare cDNA clone HF23P23 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HF23P23
Probe
GBR1822
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 669 Std Error: 0.00; Plate: 23 row: P column: 23; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp
DNA
ggcattcgattcggagcccagcccgtccggcggcgcggagccatgggcac
ggttgtggatgctcccgcagctgtgactgagaaggaggtggttgctgaga
acatgttgggtgacaagaaagtcacagtagtatttgttcttggtggtcct
ggaagtggaaagggcacacagtgtgccaacattgtggaacactttggatt
cacccatcttagtgctggagatcttttgcgcgcggagattaaatctggct
ctgagaatggaactatgattgagaacatgataaaggagggaaagattgtt
ccatcggaggtgactataaagctcttgcagcaagccatgataaagaatga
gaatgataagttcctcatcgacgggtttccaaggaatgaagagaatcgtg
ctgcgttcgagaatgttacaaaaatttcgcctgcatttgtgctattcttt
aattgttccgaggaagagatggaaagacgtcttttgggacgcaatgaggg
tagagttgatgacaacattgagactatcaggaagagattcaaagtttttg
ttgaatccagtttgcctgtgattgagtactatgacgcaaaagacaaggtt
aaaaagatcgatgctgcaaaaccaattcctgaggtgtttgaagatgtcaa
agccatttttgccccattt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BU990074
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||