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GrainGenes Sequence Report: BU991069

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Sequence
BU991069
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC157608
NCBI UniGene
Hv.3154
DB Remark
Locus Source: Hordeum vulgare
UniGene title 'CDNA clone: FLbaf102m02, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HD05N23r HD Hordeum vulgare cDNA clone HD05N23 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD05N23
Probe
GBR1682
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 593 Std Error: 0.00; Plate: 5 row: N column: 23; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
agaagaggaatcgaccatggcgacccgtatccagtttgagaacaactgtg
aagttggtgttttctccagctacaaatgcctactgtctggttgcaattgg
aggatcagagaatttctacagtacatttgagtctgagcttgcagatgtca
ttcctgtggtcaagacctctatcggtggcactagaataattggccgcctc
tgtgttggaaacaagaatggacttctcttgccacacacaaccactgatca
agagcttcagcatctgaggaactgcatacctgatcaagtggttgttcagc
gcatcgatgaaaggctgtctgcccttggcaattgcatatcctgcaatgac
catgttgcgctcacacaccctgaccttgacaaggcaactgaggagcttat
tgcagatgtgcttggggttgaggtattccgtcagacaattgcaggaaata
tccttgttgggagctactgtgcattctctaacaggggtggactggtccat
cctcacacatccattgaagatcttgatgagctgtccacactcctccaagt
ccctcttgtcgctggaaccgtgaaccgtggtagcgaggtcatt

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