GrainGenes Sequence Report: BU993564

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Sequence

BU993564

External Databases

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare

UniGene title 'Transcribed locus, moderately similar to NP_001056655.1 Os06g0126000 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Hordeum vulgare

Cultivar

Golden Promise

Clone Library

Tissue

callus

Developmental Stage

callus (5-10 mm in diameter)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HD13P04r HD Hordeum vulgare cDNA clone HD13P04 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

Clone

HD13P04

Probe

GBM1464

Remark

DB_xref: taxon:4513

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 512 Std Error: 0.00; Plate: 13 row: P column: 4; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

DNA

cggcacgaggtagaagctcagcacaaatagggtttgctccagcaggaggc
atctcagaatgctgctgcgttaaacaattaccagaacatgcttagaggct
caagcccgaatcaaagtttgcttcagcaggaggcatcgagcatcttcaaa
ggtcctacagcagtgcacagtggcattcagctggaagcatctaggtcatt
ccgtgcggctcagcttggtcaattccagcatcccatgtcgttccagcaag
gtatgccccagcaccagcataccaatttccaaggcctgggcgctagtcca
caattccagcagcatgtgatcaaccagctgctgcaagaagccaagaatac
caatagccgtgctcttgctcatcagcaacagcagcagcaacagcagcagc
agcagcagcagcagcaacaacaccgtcagcagcagcagcagcatcagcag
caacatcaacagcagcagcagtctcctagcactcccaatgcaaatggtgg
tcttgcatctgg


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