GrainGenes Sequence Report: BU995353

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Sequence

BU995353

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

male inflorescences

Developmental Stage

male inflorescences (approx. 2 mm in size), green anther stage

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HM09P17r HM Hordeum vulgare subsp. vulgare cDNA clone HM09P17 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HM09P17

Probe

GBR1698

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 602 Std Error: 0.00; Plate: 9 row: P column: 17; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

gcagacgaagaagggagaggcttttgaaggaccagagactcagggagcag
cagaggatcagtcgagaaataaaggaaaaagagcgcctggaagcagaaaa
aataataaatgatttgaaaaaagtaattaagaatgggggcaaaattcaca
ttgagggggatatgacaaaacaattcgccatggaggtggtacggagtcaa
cgggtgaaggagtttgaagagatggaaaaaaaactgcagaagcttgcaaa
gaaaatggatcacttggagagggcaaaacggcaggaggaagcaccactga
ttgaggaggccttccgaaagcgccttgaggaagaaaagatccttcatgag
catgagcagctgagagagattgagctcagcaagcaacaccacacgggtga
cttggaggagaaaaataggctttctcgattgttggagcataagaatgctt
tccaggaaagaattgtccaacgccgtgaagctgagtatagtagcctcagg
aaagagagggaagaaaggattaatcagttgatatcttcaagaaagcgtga
aagggagattgtaaggaaattgatgcattacctgaacatggaggaacaac
gg


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