Query (optional)   in Class  

GrainGenes Sequence Report: BU996424

[Submit comment/correction]

Sequence
BU996424
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC176878
NCBI UniGene
Hv.4810
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001042870.1 Os01g0314100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HM
Tissue
male inflorescences
Developmental Stage
male inflorescences (approx. 2 mm in size), green anther stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HM13I24r HM Hordeum vulgare subsp. vulgare cDNA clone HM13I24 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HM13I24
Probe
GBR1855
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 653 Std Error: 0.00; Plate: 13 row: I column: 24; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgagggcaatcatctttggaagaggatgttggtcaagttgagtta
ccggatggaggaaaacattacttgatccacacttttacgaagataagaga
atccctctcaggggttaccagagatcaatggtgaaatcaatgagcctggc
tgcaaaagtaccgcattttcactatctggaggaaataaactgtgatgctc
ttgtaaaactgaaggcatcatttcagaaggagaataaggatcatgatgtg
aagcacaccttccttccattcctcataaaatctctttctgtggcactgag
taaatatcctttgttgaacagctctttcattgaagaaactaatgaagtta
ctctcaaaggatcccacaatataggggtagctatggctaccgcacaggga
ttagtggtgccaagcatcaagaaagtccagtcactttctatattggagat
cacgaaggaactgtcaagattacacgagatggcattgcacaacagactaa
gcaccaacgatatcactggtggaaccataacgctgagcaacataggcgcc
atcggtggcaagtttgggtccccagttctcaacctacccgaggttgcgat
cattgctcttggccgcattcagaagcttcctcgctttgatgatgacgaaa
acg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.