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GrainGenes Sequence Report: BU999962

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Sequence
BU999962
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC174781
NCBI UniGene
Hv.10055
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to NP_001061119.1 Os08g0176800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HS
Tissue
embryo + scutellum
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HS16I20u HS Hordeum vulgare subsp. vulgare cDNA clone HS16I20 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HS16I20
Probe
bah63j19
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 489 Std Error: 0.00; Plate: 16 row: I column: 20; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
aaagcgacttacgatgataacttagcagttgctatataagtatacatata
tggaatcagaataactccaaatgtcatgcccatatttccaaggacctaca
ccacttacaagacactgaagactttcacgttttgacgaaaggctacgcac
tgacctgactatatatcacaacacaataacacggggaaaccattccttca
gacaactatgcaatacagaaacggctatgcgctacgacctacggctaagt
aacctatcgctggctgctgggactaggcttatcatctaccaggtgtaaca
acatccagaggacacgcaaggcatggcccgagcgaaacaaggcccttgaa
tatacatgatctcttgcgcaactatccactttcttggcaggcaggatgcc
atcacttccgtcctgttgcgattcttggctttcctttcacctcggcttcc
tggggactgtggaggtagatgggacgtctttgcgtttgc

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