GrainGenes Sequence Report: BV211333
Sequence
BV211333
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
STS
Species
Triticum aestivum
Clone Library
Wheat var Chinese Spring Genomic DNA
Data Source
genbank Release 145, Dec 15 2004
Title
BARC255 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe
BARC255
Remark
DB_xref: taxon:4565 Feature: primer_bind: On complementary strand. Feature: source: mol_type = 'genomic DNA' Locus Comment: Synonyms: XBARC255; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GTGGCGGCTTGCGGGTGGCTGAGTA; Primer B: GGGTCGGCTAGCCTTCTTTTATGTT; STS size: 230; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 52 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature
primer_bind 5 29 Location: 5..29 primer_bind 210 234 Location: complement(210..234) STS 5 234 Location: 5..234
DNA
ccatgtggcggcttgcgggtggctgagtagtattcactgcggcatctata
tggcgaatgaccactaaataaaaaaaactaccacgactaaaaaaaccaag
atcagtttaactaaaccaatgagtttactaaaccacagttaaccgatcta
attcaaataataataataataataataataataataataataataataat
aataatataaacataaaagaaggctagccgaccctccttctcggagctcc
cctgaatcaggatgttcttggctgtttgatcacgttgaatctgatcaacc
gagcagccaggtccgtcagatccttaccgctggtttcatcttgtggatga
tttttaaggtccatgactgatgggctcggggtccctttcgattggctagg
tgaaactttactgggtgaaaccgccctttccccactcgcctcatgtgcct
cgctcttttgctggcatgcttcgggg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BV211333
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||