GrainGenes Sequence Report: BV211353

Sequence

BV211353

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC1164 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC1164

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC1164; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GCGATAAGAGATGGCAAAGGAAAGG; Primer B: CAGCTTATTAAACAACGCCCGTCAA; STS size: 257; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 52 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	289	313	Location: 289..313
primer_bind	521	545	Location: complement(521..545)
STS	289	545	Location: 289..545

DNA

ctgccccccggggggctgggttcggggtgagagtgccggcattgtttttc
tctaaaaccggcgaaaagtgacgctttgggggcgcgagtggaatgatttt
ttagcgccggtgctaaaaaagtgacttgaggggcgttcctgggggcgtgg
gtagagatgcccttacccttccttttccgcgagggagtgctgaataaagt
ctcttgtaatggaaggcgttaggatatacnggagttgcgctattggcatt
ttggtacgaaaccaatttagctccgtgaaatattcgcggcgataagagat
ggcaaaggaaagggaaagctccgggaaaaggttcagagcgccactcggtt
gccgcgtatggctaagtgagagcgactctactgtcgctccctcgcgcgtt
agaagatcatacgtgcacgtcgtgttcgctgcataagtatttgtgtctga
caggcatcagataacaggcgtaaagaaaaggcaaaaggagagagagagag
agagagagagagttgaggatttgacgggcgttgtttaataagctgcgggt
gtggtggcggaacgcccgcgtttgcctggacctggacttccgatgatgac
atggggtggcgggataagacgt


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