GrainGenes Sequence Report: BV211356

Sequence

BV211356

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC1006 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC1006

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC1006; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: ATTGGATTACGCCGACGACCT; Primer B: TGGGGTAATGAAGTGTTGACGA; STS size: 229; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 53 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	176	196	Location: 176..196
primer_bind	383	404	Location: complement(383..404)
STS	176	404	Location: 176..404

DNA

ttgacagccaattcgagctcggtacccggggatcctctagagtcgacctg
caggcatgcaagcttccgcattatcgacggtcgcatgatggctcggtcag
tttccgtacatagtccggcgaacgtcgcattcagaactcggaccgacctg
tgaatccaggctttgccatgcctttattggattacgccgacgacctgcat
cgacgtcgacttcggcgccaggccatatttttttattactcactttgcat
aaattatttattatgcaacgatttattattattattattattattatttc
tggtttgcactatttcttgtgcacaggaaaatgacccagctggactatat
cctttgacgtcacctcggctggacggggggcttcgtcaacacttcattac
cccatgcgagggactttggcatcactctgccgtcctgcattggccgtgct
atgtcaccacttcggagtgccgagctctatgctccgccacctcgggaccg
ccttgggggatggaaacttgtcccgcatnaagctcggaccgggtggcaat
accgaacacattaacccagcttaaagttgcctttcatgctcaaagntttn
aaattctaacttggggttcgggtncaaccaaacatttatacnttttttgg
naaaaaaaagtggcttgggaaaaaattttccttggccaaactttgtttgg
ncaatcc


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.