GrainGenes Sequence Report: BV211364

Sequence

BV211364

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC1145 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC1145

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC1145; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GCAAAGCTCGCGATACATCAAGAT; Primer B: TGCAGGAATTCGATATCAAGCTTA; STS size: 122; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 55 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	200	223	Location: 200..223
primer_bind	298	321	Location: complement(298..321)
STS	200	321	Location: 200..321

DNA

cccacaagtggatcaccaaaacatacatcaagtggatcacgtgatatccc
attgtcaccacagataaacacggcaagacatacatcaagtgttctcaaat
ccttaaagactcaatccgacaagataaccttcaaagggaaaactcaattc
atcacaagagagtagagggggagaaacatcataagatccaactataatag
caaagctcgcgatacatcaagatcgtgccatagagagagagagagagaga
gagagagagagagagagagagagagagagagagagagagagagtcgataa
gcttgatatcgaattcctgcagccccccacgagggtggggggcgcgccta
ccccctgggcacgcctccctacctcgtggacgcctcgttgcttccttgac
gtccactccaagtctcctggattgcgtttgttccaaaaataactctcccg
aaggtttcattccgtttggtattccttttctgcgaaacactgaaataagc
aaaaaaaccagcaatttgcactgggccttcagttaataggttagtcccaa
aaataatataaaagtgcataataaaggctattaaacatccaaaacagata
atataatagcatggaacaatcaaaaaatttagatacgttggagacgtatc
aggcacgtggacccgcgtcgtgagggcaacttcggtggcccactccggcg
agagcgcggccgcagccgccatgcgcgtgctaacaaggttcatgagtgtg
gccgcaatttgcgatcgggcggaaaaggcagcccaaacggccgctgttgc
ttctcaggtggcggcaacaacatctgcctcggggatagcaactggcgaga
acggcacatcagttgtccattccgcagcggcggccttagccctgatgaag
gccgcccacgaccatgtcgaggccgcccacgccgagctcctggccgtcac
cgcacaaatcgaacgaagctt


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