GrainGenes Sequence Report: BV211403

Sequence

BV211403

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC342 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC342

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC342; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GCGTTGCTGTGGGATTTTGT; Primer B: AATGGTACGCCGAAGGGGATAG; STS size: 148; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 52 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	827	846	Location: 827..846
primer_bind	953	974	Location: complement(953..974)
STS	827	974	Location: 827..974

DNA

ctcagcacgtccaaccgtgcctctagtttcgaattcactgtttagccgtc
gcccccgtgcttcggccgccatggctgaaccgttggagctccgctcccga
gcgtgcttgcccgctccctccgtagccttaaccgcccagcgggggcgccc
cttcctgcccagaccgcccgtcgcccgcgcttctgcctctccccagctgc
acacgcccgcccgtgcagcccccgcccgctgccgcagctgttgctcgcct
gccactgctgctgcgtcccagctctactcacagttgtcgctgcgcccggt
cacgcccaaccgctggctactgcacccccacgccgcctctagctctagtc
gagcccgccagtgccctctctgtgcaggcgtgcacagccagagaggccgg
ctggctctctaccagtggcgcctggccccaacttaacttgggagcctaat
ccccctttagtactaacccacccaccactagtctagtctatgacagtggg
gccccacaatttttagactgagttaaataatctattaactagagagagag
agggagagagagagagaaacctgacaagggggccccacccaggtcgaaac
cgccgttgactagtgccacatcagtagcgggtggagaaaaaccagcgggg
gggggggtgttttgtcaggtttgggtttgttcggggggtaaaacaagttg
gaaaatagatcagggggtaaaatgaaccacccactttattcagggtagta
aaatggacttttttcttatatttgcatgtgagctacttttttactacttc
cctagttatactggatgacaccccgcgcgttgctgtgggattttgtagat
gaaatttgatgcacagagacatgtccaaaatgattatatatatatattta
tttatttatttatatactacatcaggacgaatgaccgtttccatcaattg
tactatccccttcggcgtaccattaatttcattcatgatcaaacgtctac
ccatcaaaacagaccttgtactttcagttggaatggctgtggcacaaaca
ctattgtttccctaccttgaatctcaaagaaaacaaagaaatgctgagct
tgtgcactgacttgaaacaaatagtacagttgtataaatttcttcgtcca
g


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