GrainGenes Sequence Report: BV211415

Sequence

BV211415

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC241 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC241

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC241; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GGACATGGTTTTCGGTGGGACTTTG; Primer B: TGGGCAAGCACATATGGTAAT; STS size: 185; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 50 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	269	293	Location: 269..293
primer_bind	433	453	Location: complement(433..453)
STS	269	453	Location: 269..453

DNA

aaaaaaataggatttgtaaaaaagatgggcttaaatgctacataaccaat
gaataaaaattgaagaaaaatatggatctctgttacccatataacataac
aaattatgcatgcaagtttagattttgaaagacaaacctcaatgtaaagg
ttcacaattcatgccctatgcttgctcaaacataaaattatgaacaaata
ttctcaaacgcgcacatggtattaaatgggattatgtgccatttggggca
agagtcatattttcagacggacatggttttcggtgggactttgtgttgcc
aaatttaaatgtgtgtgtgcatctaattgtagacaacaagccacaattct
ttattattattattattattattattattattattattattattattatt
attattattattattatagagcgttaaattttattaccatatgtgcttgc
ccacaacaaaaccaactaaattatggatccagcgggtctctgcaccactt
ggtaaaaatcgccacaaaaagaggggaaaagtataatctggataaatgag
aactaaatccatgcatgcaaagaaatccgacatagt


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.