GrainGenes Sequence Report: BV211418

Sequence

BV211418

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC253 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC253

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC253; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GGGAAGACACGACACGACTC; Primer B: TCGTAAGATTACCTCGGATGAAGAA; STS size: 166; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 50 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	90	109	Location: 90..109
primer_bind	230	254	Location: complement(230..254)
STS	90	254	Location: 90..254

DNA

gggtcggccccgttggcacgctagcatgacacaggtcaggcacaacacat
gctaagtgggctgtgccccggctgctaggcggggcacgcgggaagacacg
acacgactcacttatcttcttcttcttcttcttcttcttattattattat
tattattattattattattatatattaagatatttatatatagtattact
attcatcacccagggtgcaaaataagttattcttcatccgaggtaatctt
acgatcgcttcctaaataattacgtttagaatataaatagttgcatgcat
attgattcaatatgtaaaatttggtataaaaaacaaaaagttagattata
agaccagaaaaatcacattttatgtatgttttacactatatttttacatt
cataattttacgtaacataaaatatttttacgccgagtacatattttttt
acgttctatttttatgtatgaaataagacaaaatttacgaaacataaaaa
tatgatgcattaatgtcaaaatagagaaaggatgaagaataactattcct
cacccag


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