GrainGenes Sequence Report: BV211419

Sequence

BV211419

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC257 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC257

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC257; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: TGGTTGTGTGGATATCCTGCTGA; Primer B: TGCCCACTAGAATCAGAAACAATC; STS size: 269; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 50 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	424	446	Location: 424..446
primer_bind	669	692	Location: complement(669..692)
STS	424	692	Location: 424..692

DNA

aaaaaaaaaaaattagagagcgtgtatccggttccaaaattcggcttccc
cacgcaccttataataacgagaaagcccttcttcttatctcctccagctt
tctcaatctcctccagctttctcaatctagtgtccatcagcgtgtcctcc
catctccacttttttcccctctaaaaccccaacgccctcctccaatcccc
ctatcctcacagcaaatggtggtggtggtggtggtgggtacacgcggagg
agagacctcgtccctgtgatgccctgccatggctgcccgcgcgctggtcg
caccccctttgcctccaactccccctctgggtaccatgcgggcaaccccg
cgcgtcgccgccggtctcagctcagcgctgaccggccaccggggaaggtc
ccttcctggatttcatcctcatttggttgtgtggatatcctgctgatacc
gaatcttttttgtgtggttttgcttagtgacttgcttctcgcacattatt
aaagtaacagttcagagttcatgtatttatcgtgaaatcatcgtgagtga
attcttggattagtcttatgtttgctaataataataataataataataat
aataataataataataataataataataataataataataatatggaagg
cagtaagaagagttcctggattgtttctgattctagtgggcagtagaaca
gtatctttgcctgcttagatgaacggagcttcaatttcccttgtgccact
tgatcccgtggttgattggcaactcatatttattcaggcacactttgtgt
tcagtgcaattgacagaatcccccagtggcaatcttcaggtggaagca


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