GrainGenes Sequence Report: BV211422

Sequence

BV211422

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC298 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC298

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC298; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: CCCGGGTCTATTTTCCATCAT; Primer B: CGCGATAAAGGGGTTGTCAAA; STS size: 187; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 50 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	257	277	Location: 257..277
primer_bind	423	443	Location: complement(423..443)
STS	257	443	Location: 257..443

DNA

tcttgcttaaagtgttactccgttcttatgaacttaatactctagatgca
tgctggatagcggtcgatgtgtggagtaatagtagtagatgcaggtagga
gtcggtctacttttcacagacgtgatgcctatttacatgatcatacctag
atattctcataactatgctcaattctatcaattgctcgtcagtaattcgt
tcacccaccgtaatacttatgctatcttgagagaagccactagtgaaacc
tatggccccgggtctattttccatcatacaagtttccaatctattttatt
ttgcaatctttactttcaatttatatcataaaataccaaaaatatttatc
ttattattattattattattattattattattatctctatcagatctcac
tcttgcaagtgtccatgaagggtttgacaacccctttatcgcgttggttg
cgaggttcttatttgtttgtgtaggtacgaggtgactcgcgcgtggtctc
ctactggattgataccttgtttctcaaaaactaagggaaatacttacgct
gctttgctggatcaccctttcctcttcaagggaaaaccaacacatgctca
agaggtagcaaggatgtccttga


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