GrainGenes Sequence Report: BV211435
Sequence
BV211435
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
STS
Species
Triticum aestivum
Clone Library
Wheat var Chinese Spring Genomic DNA
Data Source
genbank Release 145, Dec 15 2004
Title
BARC209 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe
BARC209
Remark
DB_xref: taxon:4565 Feature: primer_bind: On complementary strand. Feature: source: mol_type = 'genomic DNA' Locus Comment: Synonyms: XBARC209; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: ACTGGTGAAGCCTCCTGCCTGTC; Primer B: GCCTCACCGACTCATCCTCTCTTTG; STS size: 145; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 52 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature
primer_bind 25 47 Location: 25..47 primer_bind 145 169 Location: complement(145..169) STS 25 169 Location: 25..169
DNA
gttgtggactcgccgtctctagccactggtgaagcctcctgcctgtccgc
cgcggtggcatccatcgcctctggggcccggtcgatcgtcgtggtccggt
gggttgcaagagagagagagagagagagagagagggagacaagacaaaga
gaggatgagtcggtgaggctctcgaacaatacgtgtggtattttataggc
cgtgctggccgccgtgagtgctggcgtcgactgttcatattgacgtgctc
gcgatgaaaacaccgaagactttgggcactccgtgagcataattgaatac
cactagt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BV211435
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||