GrainGenes Sequence Report: BV211444

Sequence

BV211444

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC277 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC277

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC277; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GGGTGGGGTAAGTTCGTCAAA; Primer B: ACGCCTGCAAGTTATAGTAGTTCT; STS size: 276; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 52 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	118	138	Location: 118..138
primer_bind	370	393	Location: complement(370..393)
STS	118	393	Location: 118..393

DNA

aaccatgctgatgaacgccatcaacttggctgcgtgggtgtttctcatga
gcgccggattgttctacgtcaatctcgataattggaccaagaacgatggg
tttctgccgaacggctggggtggggtaagttcgtcaaactgttgctcgaa
acgcgtttatataataataacgatattgtatattatatatatagacgcag
tcgtatcatcagtattattattattattattattattattattactaccg
tcgtaaaagcttcgtcgacttctgaaccattaggtgggttgatacgatac
tattattattattatactacctatcggtaaactatacgttgatgtattcg
cgtaaaataatttggttatagaactactataacttgcaggcgtttcacga
tgtcccacgcgcgatagctgaatttattatataggtacgcgtaacttcgt
gtacttaccgaaagtcaatatcgacggcgcggcgagataataataatatt
gcaaactcgcgacggttaggtttttttatttttattttttttttttggcc
ctgcgcctttgtgttaaacacacccggttaaaaaagtcggtaggtaccta
tcgatattaataatataatattatggaatcggtaaaatgctgcaggtgtt
aacggacgacgcgacggtcgcttgttgtttttacgtcgtcaaattttaag
tgaaaaaatgatattatataacattgtcatgtacgtcacggactgtattc
gatcgctattataagcgacttttaagtctgaaattactatattcctgcaa


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