GrainGenes Sequence Report: BV211455

Sequence

BV211455

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC1157 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC1157

Remark

DB_xref: taxon:4565

Feature: primer_bind: On complementary strand.

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC1157; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GACATGTTCGGTTGCCTCGGGGACTT; Primer B: ACGCTGCCCAGTCTAGATCATTTACCT; STS size: 219; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 55 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	200	225	Location: 200..225
primer_bind	392	418	Location: complement(392..418)
STS	200	418	Location: 200..418

DNA

aaataaaatatttttgtgaaaagcaattacgcaaggattttatatatatt
tatgttattctttttggaccgcactattttatgtgtgtaggtgatcgacc
ttgactgcatcacgcggtcacttcgacaaagccaacgccgtcgtcccaat
tggcataaatcggctcgcatgatcaccttgttaattgaattgccataccg
acatgttcggttgcctcggggactttggcattatcaagaaggttctacct
tgtcgggtgttcggcgcatgggccatatttcttttattactcactttgca
taaattattaatcatgcaactttttaattattattattattattattatt
attattactattatctccggcttgcactatcttctgtgcataggtaaatg
atctagactgggcagcgttgtctcctcggcgtatcgacataccacgtcac
ttcggctggactggtggcttcgtcatcacttcattaacccacacccggta
cttcggcgtcacactaccggcctgcatcggacgtgctatgtcaccacttc
ggagcgtcaagctcttcgttcttccacctcgggaccggtttgtgggctgg
gagctcatcccgctagaagctcggaccgcgtcatcaatgccgaacacatt
aaccagctaagtcgcttttatgctcaaagttttgg


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