GrainGenes Sequence Report: BV211459
Sequence
BV211459
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
STS
Species
Triticum aestivum
Clone Library
Wheat var Chinese Spring Genomic DNA
Data Source
genbank Release 145, Dec 15 2004
Title
BARC222 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe
BARC222
Remark
DB_xref: taxon:4565 Feature: primer_bind: On complementary strand. Feature: source: mol_type = 'genomic DNA' Locus Comment: Synonyms: XBARC222; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: AAATCCGGCATCTGCTGTATCCATA; Primer B: GTCCGGCCGCTGAATACTGTT; STS size: 182; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 55 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature
primer_bind 114 138 Location: 114..138 primer_bind 275 295 Location: complement(275..295) STS 114 295 Location: 114..295
DNA
acacgtacgagcgagttaaaccacgcgcatacaatacaggcggggcagta
cacacgctgccgcggcatgtcagatcaacatgcttcgccgcgtatatggt
acagtacacggaaaaatccggcatctgctgtatccatacactggatctat
ctctccatgcagaagccagctctctctctctctctctctctctctctcta
cacgcagaagccacagctctctccctctgcatgcagaagccagccttgtg
gcaccatttttgcgacgcagtatgaacagtattcagcggccggacctgcc
tgctgcgctgcatatggtgtgtgcaggcggggagattcatatctcaggag
cccgatccatcgatccgttgctccatagcaaagatcatgcacgtattttt
aggcgcccgaggcaggcaggcag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BV211459
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||