GrainGenes Sequence Report: BV211594
Sequence
BV211594
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
STS
Species
Triticum aestivum
Clone Library
Wheat var Chinese Spring Genomic DNA
Data Source
genbank Release 145, Dec 15 2004
Title
BARC1088 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe
BARC1088
Remark
DB_xref: taxon:4565 Feature: primer_bind: On complementary strand. Feature: source: mol_type = 'genomic DNA' Locus Comment: Synonyms: XBARC1088; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: TGCGACATTGCCAACATCTTAGTT; Primer B: AAGGCAGTGGTTTTCGGTTTTCTA; STS size: 120; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 52 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature
primer_bind 456 479 Location: 456..479 primer_bind 596 619 Location: complement(596..619) STS 479 596 Location: 479..596
DNA
taatttattaaaatgagaacactaatatagagagaagataaagaaaggaa
acatcataaccaacagggcttcagacaaaatatatgctgagatactaccg
caatatgaattgcttgtttgcaccttcgtaaagaaaatagtattttagag
caatttcacacacacacacacacacacacacacacacacacacacacatc
tttattgcataataaattcagagaattttttgaattgttttggcgcctga
cacgttacacaactccaccagagaaataacatcataatggttcactaaaa
cttgtcgacataggagctagcatgaaagaaagtttgtaatttgaacgatt
tgcataagttgtttctgttcgagaaaaagtcaatcccactcctcctgtga
gcccctcccattcaccctacatgtcaagcagggagttgcccttgaccttc
ctcaaaaggcagtggttttcggttttctagcgactgtcaaaagaaaatgg
catactactcccatgcattggtctaccaggaaacattcccaatttaaacc
tgagatggacagcttgaaatatacaaatatgaactttgagctaacaacta
agatgttggcaatgtcgcaagagactcaaggatgacgtgactactgacta
gagtg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BV211594
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||