GrainGenes Sequence Report: BV211609
Sequence
BV211609
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
STS
Species
Triticum aestivum
Clone Library
Wheat var Chinese Spring Genomic DNA
Data Source
genbank Release 145, Dec 15 2004
Title
BARC1123 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe
BARC1123
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'genomic DNA' Locus Comment: Synonyms: XBARC1123; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GCGGTGAAGATTAATTACGTCCAGT; Primer B: GCGGTCTTCACTGCTGCTGCTTGTATG; STS size: 126; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 52 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature
primer_bind 484 508 Location: 484..508 STS 484 613 Location: 484..>613
DNA
agggcaagtcttgtttgcttttctcaaactaaatcaaatcaaatcaggtg
cgatgggaatttctctttctttcttctttcgttggaatttttcgacatca
atctgaacttcatcagtgtatgcctctttaatctgaactttatcggtgca
cgccttgtttgcacaagtaatatactagcgcctccattccaaaatataag
acgtttttgtaggccggtaggctaaaatgtcctaccaaaacgtatatttt
gaaacagaggtaataatattctgctctatgcatgtctctaatctctatgt
aaagatgttcttctgttagagtatgctcagaacttaaagcctgcctgaca
actgcacgcaggtcaaagatgaaaattgtggtcaaccgtcgatctctaca
gcaatcaatcgttcaaaatcaccaattggtcaaaggccggtatggacaaa
tcaacagagcgaccagatagaaatttgcaaattgcggtgaagattaatta
cgtccagtacaagccaacttcctttattattattattattattattatta
ttattatcacaccatgacagcaaccacgaagacatacaagcagcagcagt
gaagacgcagccg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BV211609
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||